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#AMPLIFX IN SILICO PCR TRIANGLE SOFTWARE#
The software is available for download at and online version at. FastPCR allows for sequence file batch processing that is essential for automation. FastPCR performs efficient and complete detection of various repeat types with visual display. The program also supports the assembly of a set of contiguous sequences, consensus sequence generation, and sequence similarity and conservancy analysis. FastPCR includes various bioinformatics tools for analysis and searching of sequences, restriction I–II–III-type enzyme endonuclease analysis, and pattern searching. The in silico PCR primer or probe search includes comprehensive analyses of individual primers and primer pairs.
A program is available to design specific oligonucleotide sets for long sequence assembly by ligase chain reaction and to design multiplexed of overlapping and nonoverlapping DNA amplicons that tile across a region(s) of interest for targeted next-generation sequencing, competitive allele-specific PCR (KASP)-based genotyping assay for single-nucleotide polymorphisms and insertions and deletions at specific loci, among other features. The software provides comprehensive tools for designing primers for most PCR and perspective applications, including standard, multiplex, long-distance, inverse, real-time with TaqMan probe, Xtreme Chain Reaction (XCR), group-specific, overlap extension PCR for multifragment assembling cloning, and isothermal amplification (Loop-mediated Isothermal Amplification).
The FastPCR software is an integrated tool environment for PCR primer and probe design and for prediction of oligonucleotide properties.
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